If you haven’t already, download the Excel template for renaming raw data files here.
If you don’t have access to Microsoft Excel, you can use any other spreadsheet software such as: Apache OpenOffice™ “Calc”, which can be found at www.openoffice.org. When saving the file, ensure you save it as type “Microsoft Excel 97/2000/XP (.xls or .xlsx)”.
When applying bacteria you want your spot to look like the the spots in columns 1 to 2. Column 2 has a little too much, and 3 has too much cell material
| 1. Apply bacteria directly without any prior chemical treatment. Smear a single bacterial colony in a thin layer directly onto the MALDI target plate using a sterile toothpick. | |
| 2. Leave the Calibration spots empty. | |
| 3. Add 1 µL of 70% formic acid to each bacterial spot, let air dry. | |
| 4. Add 1 µL of MALDI matrix to each bacterial spot, let air dry. |
The MALDI plate should be properly cleaned before shipping. In order to clean the MALDI plate, use the steps below: method adapted from Freiwald & Sauer
Address:
Dr. Brian T. Murphy 900 S. Ashland Ave. M/C 870 MBRB 3114 Chicago, IL 60607
Contains:
Address:
Contains:
Use the Shipping Label found here:
Address:
c/o
Contains: